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human hepatic carcinoma hepg2 cells  (ATCC)


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    ATCC human hepatic carcinoma hepg2 cells
    Human Hepatic Carcinoma Hepg2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 30224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+hepatic+carcinoma+hepg2+cells/Hep+G2/pm42001994-58-0-14
    Average 99 stars, based on 30224 article reviews
    human hepatic carcinoma hepg2 cells - by Bioz Stars, 2026-10
    99/100 stars

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    Cell Culture:

    Article Title: Novel bionic inspired nanosystem construction for precise delivery of mRNA.
    Article Snippet: The amount of mRNA released was measured using a Nanodrop. .. Human liver L02 cells and human hepatic carcinoma HepG2 cells were provided by ATCC and cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% foetal bovine serum, streptomycin (100 U ml−1) and penicillin (100 U ml−1) (Invitrogen, CA, United States) in a Frontiers in Bioengineering and Biotechnology frontiersin.org03 37°C incubator with 5% CO2 and 95% humidity. .. Female BALB/c mice (9 weeks old) from Beijing Vital River Laboratory Animal Technology Co. (Beijing, China) were housed in a controlled environment (temperature, 22°C–24°C; humidity, 60%) under a 12-h light-dark cycle.

    Article Title: Novel bionic inspired nanosystem construction for precise delivery of mRNA
    Article Snippet: The amount of mRNA released was measured using a Nanodrop. .. Human liver L02 cells and human hepatic carcinoma HepG2 cells were provided by ATCC and cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% foetal bovine serum, streptomycin (100 U ml−1) and penicillin (100 U ml−1) (Invitrogen, CA, United States) in a 37°C incubator with 5% CO 2 and 95% humidity. .. Female BALB/c mice (9 weeks old) from Beijing Vital River Laboratory Animal Technology Co. (Beijing, China) were housed in a controlled environment (temperature, 22°C–24°C; humidity, 60%) under a 12-h light-dark cycle.

    Article Title: Only one carbon difference determines the pro-apoptotic activity of α-tocopheryl esters.
    Article Snippet: doi:10.1111/febs.16623 α-Tocopheryl succinate (TS), a redox-silent succinyl ester of natural α-Tocopherol, has emerged as a novel anti-cancer agent.. However, the underlying mechanism is unclear.. We found that the terminal dicarboxylic moiety of tocopheryl esters contributes to apoptosis induction and thus cytotoxicity.

    Modification:

    Article Title: Novel bionic inspired nanosystem construction for precise delivery of mRNA.
    Article Snippet: The amount of mRNA released was measured using a Nanodrop. .. Human liver L02 cells and human hepatic carcinoma HepG2 cells were provided by ATCC and cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% foetal bovine serum, streptomycin (100 U ml−1) and penicillin (100 U ml−1) (Invitrogen, CA, United States) in a Frontiers in Bioengineering and Biotechnology frontiersin.org03 37°C incubator with 5% CO2 and 95% humidity. .. Female BALB/c mice (9 weeks old) from Beijing Vital River Laboratory Animal Technology Co. (Beijing, China) were housed in a controlled environment (temperature, 22°C–24°C; humidity, 60%) under a 12-h light-dark cycle.

    Article Title: Novel bionic inspired nanosystem construction for precise delivery of mRNA
    Article Snippet: The amount of mRNA released was measured using a Nanodrop. .. Human liver L02 cells and human hepatic carcinoma HepG2 cells were provided by ATCC and cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% foetal bovine serum, streptomycin (100 U ml−1) and penicillin (100 U ml−1) (Invitrogen, CA, United States) in a 37°C incubator with 5% CO 2 and 95% humidity. .. Female BALB/c mice (9 weeks old) from Beijing Vital River Laboratory Animal Technology Co. (Beijing, China) were housed in a controlled environment (temperature, 22°C–24°C; humidity, 60%) under a 12-h light-dark cycle.

    Article Title: Only one carbon difference determines the pro-apoptotic activity of α-tocopheryl esters.
    Article Snippet: doi:10.1111/febs.16623 α-Tocopheryl succinate (TS), a redox-silent succinyl ester of natural α-Tocopherol, has emerged as a novel anti-cancer agent.. However, the underlying mechanism is unclear.. We found that the terminal dicarboxylic moiety of tocopheryl esters contributes to apoptosis induction and thus cytotoxicity.



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    human hepatic carcinoma hepg2 cells  (ATCC)
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    human hepg2 hepatic carcinoma cell line  (ATCC)
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    ATCC human hepg2 hepatic carcinoma cell line
    Figure 7. A higher dose of Chlorpyrifos (CPF) stimulated glycolysis and gluconeogenesis processes in human hepatic carcinoma cells line, <t>HepG2,</t> grown in normal- and high-glucose medium, respectively. mRNA levels of G6pase (A,C) and Pck1 (B,D) were determined by RT-qPCR. Data were normalized to the values obtained for CTRL (set as 1) and shown as mean ± SD (three independent replicates). One-way ANOVA, * p < 0.05, ** p < 0.01, and **** p < 0.0001.
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    Figure 7. A higher dose of Chlorpyrifos (CPF) stimulated glycolysis and gluconeogenesis processes in human hepatic carcinoma cells line, <t>HepG2,</t> grown in normal- and high-glucose medium, respectively. mRNA levels of G6pase (A,C) and Pck1 (B,D) were determined by RT-qPCR. Data were normalized to the values obtained for CTRL (set as 1) and shown as mean ± SD (three independent replicates). One-way ANOVA, * p < 0.05, ** p < 0.01, and **** p < 0.0001.
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    Figure 7. A higher dose of Chlorpyrifos (CPF) stimulated glycolysis and gluconeogenesis processes in human hepatic carcinoma cells line, HepG2, grown in normal- and high-glucose medium, respectively. mRNA levels of G6pase (A,C) and Pck1 (B,D) were determined by RT-qPCR. Data were normalized to the values obtained for CTRL (set as 1) and shown as mean ± SD (three independent replicates). One-way ANOVA, * p < 0.05, ** p < 0.01, and **** p < 0.0001.

    Journal: International journal of molecular sciences

    Article Title: Chronic Exposure to Chlorpyrifos Damages Thyroid Activity and Imbalances Hepatic Thyroid Hormones Signaling and Glucose Metabolism: Dependency of T3-FOXO1 Axis by Hyperglycemia.

    doi: 10.3390/ijms24119582

    Figure Lengend Snippet: Figure 7. A higher dose of Chlorpyrifos (CPF) stimulated glycolysis and gluconeogenesis processes in human hepatic carcinoma cells line, HepG2, grown in normal- and high-glucose medium, respectively. mRNA levels of G6pase (A,C) and Pck1 (B,D) were determined by RT-qPCR. Data were normalized to the values obtained for CTRL (set as 1) and shown as mean ± SD (three independent replicates). One-way ANOVA, * p < 0.05, ** p < 0.01, and **** p < 0.0001.

    Article Snippet: Human HepG2 hepatic carcinoma cell line was obtained from the American Type Culture Collection (ATCC).

    Techniques: Quantitative RT-PCR

    Figure 8. Chlorpyriphos (CPF) effects on FOXO1-T3 axis in human hepatic carcinoma cells line and HepG2 cultured in normal- and high-glucose condition. (A–F) Representative Western blot of extracts from HepG2 cells cultured in both normal- and high-glucose condition (NG (A–C) and HG (D–F), respectively) and exposed to different doses of CPF (as detailed in Section 4), probed with p-FOXO1Aser256 and FOXO1A. Data were normalized for GAPDH protein. Western blots were quantified by densitometry using Image Lab Software. Results were obtained from three independent experiments and expressed as mean and ± SD. One-way ANOVA, ** p < 0.01, and *** p < 0.001 vs. CTRL. ELISA assay for fT3 in HepG2 extract cultured in NG (G) and HG (H) medium and treated with different CPF doses. Data represent the mean and the SD. Data represent three independent biological replicates. One-way ANOVA, ** p < 0.01.

    Journal: International journal of molecular sciences

    Article Title: Chronic Exposure to Chlorpyrifos Damages Thyroid Activity and Imbalances Hepatic Thyroid Hormones Signaling and Glucose Metabolism: Dependency of T3-FOXO1 Axis by Hyperglycemia.

    doi: 10.3390/ijms24119582

    Figure Lengend Snippet: Figure 8. Chlorpyriphos (CPF) effects on FOXO1-T3 axis in human hepatic carcinoma cells line and HepG2 cultured in normal- and high-glucose condition. (A–F) Representative Western blot of extracts from HepG2 cells cultured in both normal- and high-glucose condition (NG (A–C) and HG (D–F), respectively) and exposed to different doses of CPF (as detailed in Section 4), probed with p-FOXO1Aser256 and FOXO1A. Data were normalized for GAPDH protein. Western blots were quantified by densitometry using Image Lab Software. Results were obtained from three independent experiments and expressed as mean and ± SD. One-way ANOVA, ** p < 0.01, and *** p < 0.001 vs. CTRL. ELISA assay for fT3 in HepG2 extract cultured in NG (G) and HG (H) medium and treated with different CPF doses. Data represent the mean and the SD. Data represent three independent biological replicates. One-way ANOVA, ** p < 0.01.

    Article Snippet: Human HepG2 hepatic carcinoma cell line was obtained from the American Type Culture Collection (ATCC).

    Techniques: Cell Culture, Western Blot, Software, Enzyme-linked Immunosorbent Assay